Bioretention systems use plants and soil media to treat pollutants in stormwater. Biochar can be an effective amendment to bioretention soil media for removing pollutants due to its adsorption properties. This study evaluates biochar as an amendment to bioretention soil media for the removal of contaminants in stormwater. Columns with varying ratios of biochar to bioretention soil media (0%, 25%, and 50% biochar) were tested to evaluate the removal efficiencies of copper, zinc, phosphorus, nitrogen, and microplastics from stormwater. Five trials were conducted with stormwater collected from parking lot runoff. Results show that the addition of biochar in bioretention systems improves the removal efficiency of zinc (average of 79% in columns with 50% biochar compared to 54% in columns without biochar) and copper (average of 73% in columns with 50% biochar compared to 25% in columns without biochar). Stormwater effluent from the biochar-amended columns also had significantly lower turbidity (21 NTU) than that of the control columns (58 NTU). Consistent leaching of nitrogen and phosphorus species was evident in the control as well as the biochar-amended columns, with average removal efficiencies of -1,114% for total nitrogen and -703% for total phosphorus. Microplastics were effectively removed by all columns, regardless of biochar amendment, with an average removal of 86%. This study indicates that biochar has the potential to reduce metal concentrations in stormwater when used as an amendment to bioretention systems but has a limited impact on nutrients and microplastics.