The treatment of reverse osmosis concentrate generated from urban industrial sewage for resource recovery has been hot. In this research, a biofilm reactor was constructed by combining sycamore deciduous biochar, shell powder, and polyurethane sponge loaded with Zobellella denitrificans sp. LX16. For ammonia nitrogen (NH4+-N), calcium (Ca2+), chemical oxygen demand (COD), cadmium (Cd2+), and tetracycline (TC), the removal effi-ciencies were 98.69 %, 83.95 %, 97.26 %, 98.34 %, and 69.12 % at a hydraulic retention time (HRT) of 4 h, pH of 7.0, and influent salinity, Ca2+, and TC concentrations of 1.0, 180.0, and 3.0 mg/L, respectively. The biofilm reactor packing has a three-dimensional structure to ensure good loading of microorganisms while promoting electron transfer and metabolic activity of microorganisms and increasing the pollutant tolerance and removal efficiency. The reactor provides a practical reference for the sedimentation of reverse osmosis concentrate to remove Cd2+ and TC by microbial induced calcium precipitation (MICP).