Applying soil amendments such as biochar has been proposed as a means to improve soil properties and reduce greenhouse gas emission from agricultural soils. The objective of this study was to evaluate and compare the effects of biochar and straw application on rice yield, select soil properties, global warming potential (GWP), and greenhouse gas intensity (GHGI) over the 3-year period. The study treatments included control (CN), barley straw biochar (BC, 2,000 kg ha(-1)), barley straw (BS, 2,000 kg ha(-1)), and BC+BS (each 1,000 kg ha(-1)). During the rice-growing season, significant interactive effects of BC and BS treatment were observed in major soil properties. The average rice yields in BC, BS, and BC+BS treatments increased by 4.6%, 4.7%, and 18.7%, compared with CN treatment, respectively. Also, BC+BS treatment produced relatively stable yield during the 3-year study. Both BC and BS applications, either alone or in combination, lowered soil bulk density values compared to the CN treatment. Further, BC application produced a low total CH4 flux than other treatments. The total produced GWP over the 3 years occurred in the order: BS >= CN > BC+BS > BC treatment. These results clearly demonstrate the advantages of biochar and straw applications in improving rice yield and soil fertility while reducing GWP and GHGI.